Sulfur regulation of the sulfate transporter genes sutA and sutB in Penicillium chrysogenum.
نویسندگان
چکیده
Penicillium chrysogenum uses sulfate as a source of sulfur for the biosynthesis of penicillin. Sulfate uptake and the mRNA levels of the sulfate transporter-encoding sutB and sutA genes are all reduced by high sulfate concentrations and are elevated by sulfate starvation. In a high-penicillin-yielding strain, sutB is effectively transcribed even in the presence of excess sulfate. This deregulation may facilitate the efficient incorporation of sulfur into cysteine and penicillin.
منابع مشابه
Sulfate transport in Penicillium chrysogenum: cloning and characterization of the sutA and sutB genes.
In industrial fermentations, Penicillium chrysogenum uses sulfate as the source of sulfur for the biosynthesis of penicillin. By a PCR-based approach, two genes, sutA and sutB, whose encoded products belong to the SulP superfamily of sulfate permeases were isolated. Transformation of a sulfate uptake-negative sB3 mutant of Aspergillus nidulans with the sutB gene completely restored sulfate upta...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 66 10 شماره
صفحات -
تاریخ انتشار 2000